Journal article
Dysregulated miR-124-3p in endometrial epithelial cells reduces endometrial receptivity by altering polarity and adhesion
W Zhou, M Van Sinderen, K Rainczuk, E Menkhorst, K Sorby, T Osianlis, M Pangestu, L Santos, L Rombauts, A Rosello-Diez, E Dimitriadis
Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD SCIENCES | Published : 2024
Abstract
The endometrium undergoes substantial remodeling in each menstrual cycle to become receptive to an implanting embryo. Abnormal endometrial receptivity is one of the major causes of embryo implantation failure and infertility. MicroRNA-124-3p is elevated in both the serum and endometrial tissue of women with chronic endometritis, a condition associated with infertility. MicroRNA-124-3p also has a role in cell adhesion, a key function during receptivity to allow blastocysts to adhere and implant. In this study, we aimed to determine the function of microRNA-124-3p on endometrial epithelial adhesive capacity during receptivity and effect on embryo implantation. Using a unique inducible, uterine..
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Awarded by Hudson Institute of Medical Research
Funding Acknowledgements
We are grateful to Ms Judi Hocking and Ms Emily-jane Bromley for orchestrating the tissue collection and the women who donated the endometrial tissue and embryos. We are also grateful to Dr. Meaghan Griffiths (Monash University) for technical assistance, Dr. Jeremy Cottrell (University of Melbourne) forthe assistance of transepithelial electrical resistance assay and Biological Optical Microscopy Platform (University of Melbourne) for the assistance of confocal imaging. We thank Single Cell and High Throughput Genomics Centre based at The Hudson Institute of Medical Research for the assistance of Fluidigm BioMark HD analysis. This work was supported by a National Health and Medical Research Council of Australia Project Grant (APP1120689) to E.D. and L.R. and a senior research fellowship to E.D. (#550905) . W.Z. was supported by an Early Career Researcher Grant, Department of Obstetrics and Gynaecology fellowship and the Rowden White Trust Foundation (University of Melbourne) .